Proliferation and Cell Cycle models have been updated to better fit data with missing peaks.Whyte, Richard Halpert, Łukasz Kreft, James Collier, Alexander Botzki, Josef Spidlen, Stéphanie Humblet-Baron, Adrian Liston. 'Together, AutoSpill and AutoSpread remove limiting constraints of traditional compensation methods, easing the preparation of compensation controls in high-parameter flow cytometry, making errors less likely, and facilitating the practical implementation of ultra high-parameter flow cytometry.' -Roca, et alĪutoSpill: a method for calculating spillover coefficients in high-parameter flow cytometry.Īuthors: Carlos P. Our exclusive AutoSpread algorithm provides a Spillover Spreading Matrix (SSM) by calculating spillover spreading coefficients with linear models, removing the need for well-defined positive and negative populations in single-color controls. ![]() ![]() AutoSpill compensation: AutoSpill is a new algorithm for calculating spillover and producing a compensation matrix developed and incorporated into FlowJo v10.7 in collaboration with researchers from the Vlaams Instituut voor Biotechnologie in Belgium and the the Babraham Institute in Cambridge, UK.
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